DNA----transcription----RNA----translation---Protein
One gene - one protein ( for now)
tissue to tissue variation, time to time variation, subject to subject variation
what is measured ? goal : the number of mRNA molecules for
each gene (or squence tag) in the sample cells
Image data : use laser scanner to obtain
light intensity at each pixel location
Eventually converted and "treated"
as a measure of abundance of mRNA ( in a relative sense)
Example :
Yeast Diauxic shift micro-array data (see science article)
Ribosomal proteins
Aging genes affymetrics data (see science article)
What is microarray ? http://www.nhgri.nih.gov/DIR/LCG/15K/HTML/aboutmicro.html